Methogo, Ruth Menque published the artcileIdentification of liver protein targets modified by tienilic acid metabolites using a two-dimensional Western blot-mass spectrometry approach, Product Details of C13H8Cl2O4S, the main research area is liver protein tienilic acid metabolite mass spectrometry electrophoresis immunoblotting.
A combined approach based on two-dimensional electrophoresis-immuno-blotting and nanoliquid chromatog. coupled online with electrospray ionization mass spectrometry (nLC-MS/MS) was used to identify proteins modified by a reactive intermediate of tienilic acid (TA). Liver homogenates from rats exposed to TA were fractionated using ultra centrifugation; four fractions were obtained and subjected to 2D electrophoresis. Following transfer to PVDF membranes, modified proteins were visualized after India ink staining, using an anti-serum raised against TA and ECL detection. Immuno-reactive spots were localized on the PVDF membrane by superposition of the ECL image, protein spots of interest were excised, digested on the membrane with trypsin followed by nLC-MS/MS anal. and protein identification. A total of 15 proteins were identified as likely targets modified by a TA reactive metabolite. These include selenium binding protein 2, senescence marker protein SMP-30, adenosine kinase, Acy1 protein, adenosyl-homocysteinase, capping protein (actin filament), protein disulfide isomerase, fumarylacetoacetase, arginase chain A, keto-hexokinase, proteasome endopeptidase complex, triosephosphate isomerase, superoxide dismutase, DNA-type mol. chaperone hsc73 and malate dehydrogenase.
International Journal of Mass Spectrometry published new progress about Autoimmune hepatitis (drug-induced). 40180-04-9 belongs to class benzothiophene, name is 2-(2,3-Dichloro-4-(thiophene-2-carbonyl)phenoxy)acetic acid, and the molecular formula is C13H8Cl2O4S, Product Details of C13H8Cl2O4S.
Referemce:
Benzothiophene – Wikipedia,
Benzothiophene | C8H6S – PubChem